中国烟草科学Issue(2)：41-44,4.DOI:10.3969/j.issn.1007-5119.2013.02.009
TMV拮抗细菌A3的培养基优化研究
Medium Optimization of Antagonistic Bacteria A3 Strain Against TMV
战徊旭 1申莉莉 1韩志忠 2杨金广 1钱玉梅 1王盼 1王凤龙1
作者信息
- 1. 中国农业科学院烟草研究所，烟草行业烟草病虫害监测与综合治理重点实验室，青岛266101
- 2. 山东青岛烟草有限公司，青岛266071
- 折叠
摘要
Abstract
The experiments with one factor and orthogonal design were conducted to optimize medium of antagonistic bacteria A3 strain against TMV (Pseudomonas putida) with mass growth (OD600 value) as the index. A systematic study of carbon sources, nitrogen sources and inorganic chemicals was carried out. Carbon source“wheat bran”, nitrogen source“yeast extract powder”and inorganic chemical“CaCl2, CoCl2, MgSO4, FeSO4, KH2PO4”, were beneficial to the growth of A3. The results of L18(37) orthogonal tests showed that optimal medium menu included 15g wheat bran,15g yeast extract powder, 2.5g CaCl2, 0.5g CoCl2, 0.8g MgSO4, 0.5g FeSO4, 0.8g KH2PO4 and 1000mL distilled water, pH 7.0. The number of live bacteria could be 3.234×1012 cfu/mL in the optimized medium and the number increased by one orders of magnitude than original NB medium.关键词
TMV/拮抗细菌/培养基优化Key words
TMV/antagonistic bacteria/medium optimization分类
农业科技引用本文复制引用
战徊旭,申莉莉,韩志忠,杨金广,钱玉梅,王盼,王凤龙..TMV拮抗细菌A3的培养基优化研究[J].中国烟草科学,2013,(2):41-44,4.
