中国烟草科学Issue(2)：49-53,5.DOI:10.3969/j.issn.1007-5119.2013.02.011
放线菌F8对烟草黑胫病的拮抗作用及其产酶活性
Antagonism Effect and Enzyme-producing Comparison of Actinomycetes F8 against Tobacco Black Shank
摘要
Abstract
The strain F8, who isolated from rhizosphere of healthy tobacco, was an Actinomycete. In order to evaluate its application potential against tobacco black shank, tests for its inhibition activity, enzyme activity and effect on disease control were conducted in this study. The results showed that strain F8 had a strong antagonistic effect on P. parasitica var. nicotianae. The hyphal treated with culture filtrate of strain F8 sprouted abnormally and died. Besides these, strain F8 had enzyme-producing character of chitinase, cellulase and protease. After inoculated 30 d, it was founded that strain F8 could reduce the incidence of tobacco black shank by 70.3%in glasshouse experiments. According to the morphological, cultural, physiological and biochemical characteristics and 16S rDNA sequence, strain F8 was identified as Streptomyces achromogenes subsp. streptozoticus.关键词
烟草/黑胫病/不产色链霉菌/酶活/生物防治Key words
tobacco/black shank/Streptomyces achromogenes subsp. streptozoticus/enzyme-producing/biocontrol分类
农业科技引用本文复制引用
王静,孔凡玉,张成省,冯超..放线菌F8对烟草黑胫病的拮抗作用及其产酶活性[J].中国烟草科学,2013,(2):49-53,5.基金项目
中国烟草总公司重点项目“全国烟草有害生物调查研究”（110200902065）；山东省烟草专卖局项目“烟草主要病虫害生物防治技术体系”、“山东基本烟田土壤综合治理研究” （）
