中国烟草科学Issue(3)：55-59,5.DOI:10.3969/j.issn.1007-5119.2013.03.11
烤烟CMV抗性基因QTL定位
QTL Analysis of the Resistance Gene to CMV in Flue-cured Tobacco
摘要
Abstract
In order to obtian gene closely linked with CMV and cultivate resistant varieties, BC1 segregating population was generated from Taiyan-8 (resistant) and NC82 (susceptible) as parents. The BC1 population was used for virus artificial inoculation. 167 polymorphism markers were selected from 2317 SSR markers distributed in 24 tobacco linkage populations and then 62 SSR markers with stability and significant polymorphism were obtained. A tobacco genetic linkage map was constructed according to virus test results of 288 BC1. A QTL related to resistance with LOD equal to 2.6 was obtianed through WinQTLCart2.5 analysis. Our results showed that multiple genes caused the resistance of Taiyan-8;marker 53735 was closely linked to resistance QTL with the genetic distance of 0.1 cM, which could explain 13%of the variation.关键词
烟草/CMV/SSR/抗性基因Key words
tobacco/CMV/SSR/resistance gene分类
农业科技引用本文复制引用
文轲,杨爱国,张志明,任民,蒋彩虹,申莉莉,程立锐,耿锐梅,陈小翠,冯莉..烤烟CMV抗性基因QTL定位[J].中国烟草科学,2013,(3):55-59,5.基金项目
中国烟草总公司科技重大专项项目“烟草遗传图谱构建和重要抗病基因精细定位”[110201201003（JY-03）]；中国烟草总公司科技重点项目“优质抗病烤烟新品种选育及育种技术研究”（110201002002）；公益性行业（农业）科研专项项目“烟草增香减害关键技术研究与示范” （）
