中国烟草科学2015，Vol.36Issue(5)：85-89,5.DOI:10.13496/j.issn.1007-5119.2015.05.016
基于响应面分析法对烟叶中果胶测定方法的优化
The Optimization of the Method of Tobacco Pectin Determination Based on the Response Surface Methodology
摘要
Abstract
In order to optimize the method of determining tobacco pectin using enzymolysis-flow method, reaction temperature, enzymatic hydrolysis time and buffer pH were studied through the Box-Benhnken experimental design and response surface methodology. The results showed that reaction temperature had the greatest impact on the determination of pectin, followed by buffer pH, and the enzymatic hydrolysis time comes last. The optimal enzymatic hydrolysis conditions were pH 3.8, time 1.9 h and 56℃. Under this condition, the linear relationship of pectin was optimal (R2=0.999), with the recovery of pectin in tobacco ranged from 99.2% to 100.9%, and theRSD (n=6) was less than 3%. This suggested that the optimized enzymatic hydrolysis conditions used by the Box-behnken design and response surface analysis have good precision and accuracy and are suitable for testing large number of samples.关键词
响应面分析/连续流动法/烟叶果胶/定量测定Key words
response surface methodology/continuous flow method/tobacco pectin/quantitative determination分类
轻工纺织引用本文复制引用
白晓莉,侯英,张朗,潘纯祥,陶自伟,刘静,杨蕾..基于响应面分析法对烟叶中果胶测定方法的优化[J].中国烟草科学,2015,36(5):85-89,5.基金项目
中国烟草总公司科技重大专项项目"再造烟叶工业应用的特色工艺研究"[110201301019(ZZ-08)] （ZZ-08）
云南中烟工业有限责任公司技术中心项目"造纸法再造烟叶大分子物理处理技术研究"(JSZX2014JC14) （JSZX2014JC14）
