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基于 SSR 荧光标记分析我国主要审(认)定烤烟及白肋烟品种遗传多样性

陈雅琼 王卫峰 丁安明 宗鹏 李凤霞 张磊 孙玉合

中国烟草学报Issue(2):98-105,8.
中国烟草学报Issue(2):98-105,8.DOI:10.3969/j.issn.1004-5708.2013.02.018

基于 SSR 荧光标记分析我国主要审(认)定烤烟及白肋烟品种遗传多样性

Analysis of genetic diversity in national listed tobacco varieties using SSR fluorescent marker data

陈雅琼 1王卫峰 2丁安明 1宗鹏 1李凤霞 2张磊 1孙玉合2

作者信息

  • 1. 中国农业科学院烟草遗传改良与生物技术重点开放实验室/中国农业科学院烟草研究所,山东省青岛市崂山区科苑经四路 11号 266101
  • 2. 中国农业科学院研究生院,北京 100081
  • 折叠

摘要

Abstract

Genetic diversity of 63 flue-cured and 8 burley tobacco varieties in China’s national list were analyzed using capillary electrophoresis with fluorescent detection technology. 36 pairs of SSR primers were selected to amplify which generated 205 DNA bands with highly polymorphic. Dice genetic similarity coefficient, calculated by NTSYS-pc2.10e software, ranged from 0.223 to 0.924, with an average of 0.658. It was concluded that genetic basis of present cultivated varieties in China was narrow resulting in poor genetic diversity.

关键词

烟草/审(认)定品种/SSR 荧光标记/遗传多样性

Key words

tobacco/certificated varieties/SSR fluorescent markers/genetic diversity

分类

农业科技

引用本文复制引用

陈雅琼,王卫峰,丁安明,宗鹏,李凤霞,张磊,孙玉合..基于 SSR 荧光标记分析我国主要审(认)定烤烟及白肋烟品种遗传多样性[J].中国烟草学报,2013,(2):98-105,8.

基金项目

国家烟草专卖局项目“》生烟草资源评价与种质创新” ()

中国烟草学报

OA北大核心CSCDCSTPCD

1004-5708

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