中国烟草学报Issue(3)：54-60,7.DOI:10.3969/j.issn.1004-5708.2013.03.011
不同养地方式下烤烟根系差异蛋白质组学分析
Analysis of root differential proteomics in flue-cured tobacco from soils under different management methods
尤垂淮 1唐莉娜 2陈冬梅 3陈顺辉 1黄锦文 2周阳 3张重义 1林文雄1
作者信息
- 1. 福建农林大学农业生态研究所，福州 350002
- 2. 福建农林大学烟草研究所，福州 350002
- 3. 福建省烟草专卖局烟草农业科学研究所，福州350003
- 折叠
摘要
Abstract
Root differential proteomics was employed to explore the impact of different soil management methods on growth of flue-cured tobacco (Nicotina tobaccum L). Results showed that a total 39 protein spots in tobacco root at late vigorous growing stage displayed significantly different expression as compared to control. 24 protein spots were identified by LC-MS/MS analysis and database searching. Among which, two proteins related to aroma formation, i.e. transketolase, GDP-mannose 3',5'-epimerase (GME) were up-regulated under treatment of rice straw returned to field (RS), which was helpful to enhance tobacco quality. While under AS treatment, they were down-regulated with reduced tobacco quality. Other proteins involved in signal transduction, translocation of solute, protein metabolism, and energy metabolism were also identified. They were very important for growth development and all up-regulated under both RS and AS treatments. While under WF treatment they were all down-regulated and thus tobacco growth was weak. In conclusion, RS treatment was in favour of boosting growth of tobacco, increasing yield and quality, and gaining better economic benefit.关键词
烤烟/双向电泳/2-DE图谱/差异蛋白质组学Key words
flue-cured tobacco/two-dimensional electrophoresis/2-DE maps/differential proteomics分类
轻工纺织引用本文复制引用
尤垂淮,唐莉娜,陈冬梅,陈顺辉,黄锦文,周阳,张重义,林文雄..不同养地方式下烤烟根系差异蛋白质组学分析[J].中国烟草学报,2013,(3):54-60,7.
