中国药房Issue(27)：3836-3837,3838,3.DOI:10.6039/j.issn.1001-0408.2015.27.33
HPLC法测定硫磺熏蒸与未熏蒸北沙参中5种香豆素类成分的含量
Contents Determination of 5 Coumarins in Sulphur-fumigated and No Sulphur-fumigated Glehnia littoralis by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for the contents determination of 5 coumarins in sulphur-fumigated and no sul-phur-fumigated Glehnia littoralis,and investigate the effect of sulphur fumigation on the coumarins in G. littoralis. METHODS:HPLC was performed on the column of Hypersil ODS C18 with mobile phase of acetonitrile-0.1% phosphoric acid (gradient elu-tion)at a flow rate of 1.0 ml/min,detection wavelength was 295 nm,column temperature was 30 ℃,and the volume was 20 μl. RESULTS:The linear range was 0.121 5-12.15 μg/L for psoralen,0.389-38.9 μg/L for xanthotoxin,0.055-5.5 μg/L for bergapten, 0.070 5-7.05 μg/L for imperatorin and 0.092 5-9.25 μg/L for isoimperatorin(r≥0.999 7);RSD of precision test was lower than 1.0,RSDs of reproducibility and stability tests were lower than 3.0;recovery was 98.01%-101.97%(RSD<3.0%,n=6). CON-CLUSIONS:The content of coumarins in G. littoralis was obviously decreased after sulphur fumigation. the method is simple and accurate,and canbe used for the quality control of sulphur-fumigated and no sulphur-fumigated G. littoralis.关键词
高效液相色谱法/硫磺熏蒸/北沙参/香豆素/含量测定Key words
HPLC/Sulphur fumigation/Glehnia littoralis/Coumarins/Content determination分类
医药卫生引用本文复制引用
牛韬..HPLC法测定硫磺熏蒸与未熏蒸北沙参中5种香豆素类成分的含量[J].中国药房,2015,(27):3836-3837,3838,3.基金项目
山东省中医药科技发展计划项目 （）
