中国药房Issue(28)：3947-3949,3950,4.DOI:10.6039/j.issn.1001-0408.2015.28.20
舟山黄海葵粗提物抗人肺癌SPC-A1细胞的活性研究
Study on the Antitumor Activity of Anthopleura xanthogrammica Crude Extract on SPC-A1 Cells
张亚茹 1罗李王 1杨最素 1赵玉勤 1余方苗 1王飞 1丁国芳1
作者信息
- 1. 浙江海洋学院食品与医药学院/浙江省海洋生物医用制品工程技术研究中心，浙江舟山 316022
- 折叠
摘要
Abstract
OBJECTIVE:To study the antitumor activity of Anthopleura xanthogrammica crude extract on human lung cancer SPC-A1 cells in vitro. METHODS:A. xanthogrammica crude extract obtained by the methods of repeated freezing and thawing,ac-etone precipitation. After treated with crude extract 0(blank control),0.625,1.25 and 2.5 mg/ml for 24,48 and 72 h,the activity of SPC-A1 cells were measured by MTT assay. The growth inhibition rate and IC50 were also calculated. 24 h later,the morphologi-cal changes of SPC-A1 cells were observed by HE staining and AO/EB fluorescence staining. RESULTS:MTT assay showed that A. xanthogrammica crude extract has significant inhibitory effect on the proliferation of human lung cancer SPC-A1 cells;with the increasing of the concentration and the extension of the time,the inhibitory rate was increased. Its 24 h,48 h ,72 h IC50 were 1.81,1.32 and 1.18 mg/ml. HE staining and AO/EB staining appeared obvious morphological changes of apoptosis that cell mor-phology narrowed,vacuoles arose in the cytoplasm,karyopyknosis and part of nuclear disappearance occurred. CONCLUSIONS:A. xanthogrammica crude extract has an inhibitory effect on the proliferation of human lung cancer SPC-A1 cells.关键词
黄海葵粗提物/SPC-A1细胞/增殖/凋亡Key words
Anthopleura xanthogrammica crude extract/SPC-A1 cells/Proliferation/Apoptosis分类
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张亚茹,罗李王,杨最素,赵玉勤,余方苗,王飞,丁国芳..舟山黄海葵粗提物抗人肺癌SPC-A1细胞的活性研究[J].中国药房,2015,(28):3947-3949,3950,4.
