中国药房Issue(28)：3967-3969,3.DOI:10.6039/j.issn.1001-0408.2015.28.26
星点设计-效应面法优化重楼皂苷类成分的提取工艺Δ
Optimization of the Extraction Technology of Saponins from Paris polyphylla with Central Composite De-sign-response Surface Method
摘要
Abstract
OBJECTIVE:To optimize extraction technology of saponins from Paris polyphylla. METHODS:Using paris sapo-nin Ⅰ,paris saponin Ⅱand Paridis total saponin as dependent variables,using ethanol volume fraction,extraction time and sol-vent amount as independent variables,through multiple linear regression and binomial fitting,the extraction technology was opti-mized with response surface method and predicted. RESULTS:The optimized extraction technology of saponins from P. polyphylla was as follows as 10-fold of 80% ethanol,2 times reflux extraction,100 min each time. Under the extraction technology,the ex-traction rates of paris saponinⅠwere 85.4%,82.7% and 87.1%;those of paris saponinⅡwere 85.9%,81.3% and 83.6%;and those of Paridis total saponin were 89.5%,92.1% and 90.3%(all RSD<2.0%). Measured value was 0.964 9,predicted value was 0.986 0 and deviation rate was 2.14%. CONCLUSIONS:The central composite design-response surface method is simple and reliable for the optimization of extraction technology of saponins from P. polyphylla.关键词
星点设计/效应面法/重楼/重楼皂苷Ⅰ/重楼皂苷Ⅱ/重楼总皂苷/提取工艺Key words
Central composite design/Response surface method/Paridis polyphylla/Paris saponinⅠ/Paris saponin Ⅱ/Pari-dis total saponin/Extraction technology分类
医药卫生引用本文复制引用
鞠建峰,朱宗敏,杜忠科..星点设计-效应面法优化重楼皂苷类成分的提取工艺Δ[J].中国药房,2015,(28):3967-3969,3.基金项目
山东省中医药科技发展计划项目 （）
