中国药房Issue(30)：4243-4244,2.DOI:10.6039/j.issn.1001-0408.2015.30.23
HPLC法测定芪黄胶囊中青藤碱的含量
Content Determination of Sinomenine in Qihuang Capsule by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for the content determination of sinomenine in Qihuang capsule. METHODS:HPLC was performed on the column of welch C18-AQ with mobile phase of methanol-phosphate buffer(gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 264 nm,column temperature was 30 ℃ and volume injection was 10 μl. RESULTS:The linear range of sinomenine was 0.200 3-10.016 0 μg(r=0.999 8),RSDs of precision,stability and reproducibility tests were lower than 1.0%,recovery was 98.80%-100.94%(RSD=0.79%,n=6). CONCLUSIONS:The method is simple,accurate and re-producible,and can be used for the content determination of sinomenine in Qihuang capsule.关键词
芪黄胶囊/青藤碱/含量/高效液相色谱法Key words
Qihuang capsule/Sinomenine/Content/HPLC分类
医药卫生引用本文复制引用
阳勇,罗维早,梁旭明,秦伟瀚,卿大双,王德江,王颖..HPLC法测定芪黄胶囊中青藤碱的含量[J].中国药房,2015,(30):4243-4244,2.基金项目
“重大新药创制”科技重大专项（No.2013ZX09101108）；重庆市卫生局中医药科技项目 （）
