中国药房Issue(30)：4262-4263,4264,3.DOI:10.6039/j.issn.1001-0408.2015.30.30
黄金膏中盐酸小檗碱的定性鉴别与含量测定Δ
Qualitative Identification and Content Determination of Berberine Hydrochloride in Huangjin Paste
摘要
Abstract
OBJECTIVE:To establish a method for the qualitative identification and content determination of berberine hydro-chloride in Huangjin paste. METHODS:TLC was adopted for the qualitative identification of berberine hydrochloride and HPLC was conducted for the content determination of berberine hydrochloride in preparation. The column was Symmetry Shield Rp-18 with mobile phase of acetonitrile-0.1% phosphoric acid(50∶50,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 265 nm,column temperature was 30 ℃ and injection volume was 10 μl. RESULTS:TLC spots of berberine hydrochloride in prepara-tion were clear and well-separated. The linear range of berberine hydrochloride was 2.5-20.0 μg/ml(r=0.999 0);RSDs of preci-sion,stability and reproducibility tests were lower than 3%;recovery was 97.7%-102.1%(RSD=1.68%,n=6). CONCLUSI-ONS:The method is simple,accurate and reproducible,and can be used for the qualitative identification and content determination of berberine hydrochloride in Huangjin paste.关键词
黄金膏/盐酸小檗碱/薄层色谱法/高效液相色谱法Key words
Huangjin paste/Berberine hydrochloride/TLC/HPLC分类
医药卫生引用本文复制引用
陈良,刘炜,葛朝伦,田红林..黄金膏中盐酸小檗碱的定性鉴别与含量测定Δ[J].中国药房,2015,(30):4262-4263,4264,3.基金项目
新疆医科大学附属中医医院院级课题 （）
