中国药房Issue(30)：4264-4265,4266,3.DOI:10.6039/j.issn.1001-0408.2015.30.31
毛鸡骨草种子的HPLC指纹图谱研究
Study on the HPLC Fingerprint of Abrus mollis Seed
雷朝天 1唐哲 2甄汉深3
作者信息
- 1. 国药控股广西有限公司，南宁 545005
- 2. 解放军第303医院，南宁 454150
- 3. 广西中医药大学药学院，南宁 530001
- 折叠
摘要
Abstract
OBJECTIVE:To establish the HPLC fingerprint of Abrus mollis seed. METHODS:HPLC was performed on the column of Inertsil ODS-3 C18 with mobile phase of acetonitrile-0.1% phosphoric acid(gradient elution)at flow rate of 1.0 ml/min, detection wavelength was 270 nm,column temperature was 30 ℃ and volume injection was 10 µl. With the reference of abrine, 10 batches of A. mollis seed were analyzed,and similarity evaluation was performed by using chromatographic fingerprint of TCM. RESULTS:There were totally 12 common peaks,and the similarity was more than 0.9. According to the verification,the finger-print of A. mollis seed has good consistency with the reference fingerprint. CONCLUSIONS:The established fingerprint can pro-vide reference for the identification and quality evaluation of A. mollis seed.关键词
毛鸡骨草种子/高效液相色谱法/指纹图谱Key words
Abrus mollis seed/HPLC/Fingerprint分类
医药卫生引用本文复制引用
雷朝天,唐哲,甄汉深..毛鸡骨草种子的HPLC指纹图谱研究[J].中国药房,2015,(30):4264-4265,4266,3.
