中国药房Issue(30)：4274-4276,3.DOI:10.6039/j.issn.1001-0408.2015.30.35
HPLC法同时测定四环素可的松眼膏中盐酸四环素和醋酸可的松的含量
Simultaneous Determination of Tetracycline Hydrochloride and Cortisone Acetate in Cortisone Tetracycline Eye Ointment by HPLC
刘映倩 1曾杰 1邓贵福 1李绪伦 1伍彬2
作者信息
- 1. 重庆市食品药品检验所/重庆市药物过程与质量控制工程技术研究中心，重庆 401121
- 2. 重庆医药高等专科学校，重庆 401331
- 折叠
摘要
Abstract
OBJECTIVE:To establish a method for the simultaneous determination of tetracycline hydrochloride and cortisone acetate in Cortisone tetracycline eye ointment. METHODS:HPLC was performed on the column of Phenomenex C18 and shimaduz GL C18 with mobile phase of 0.01 mol/L Sodium dodecyl sulfate solution(adjusted to pH 2.5 with phosphoric acid)-acetonitrile(60∶40,V/V)at flow rate of 1.0 ml/min,detection wavelength was 254 nm,column temperature was 30 ℃,and the injection volume was 20 μl. RESULTS:The linear range was 11.36-227.18 μg/ml for tetracycline hydrochloride(r=0.999 9)and 11.11-222.21 μg/ml for cortisone acetate(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 1.2%;recoveries were 96.89%-100.67%(RSD=1.1%,n=9)and 100.04%-101.02%(RSD=0.3%,n=9),respectively. CONCLUSIONS:The method is simple,accurate and specific,and can accurately determine the contents of tetracycline hydrochloride and cortisone acetate in Corti-sone tetracycline eye ointment.关键词
四环素可的松眼膏/高效液相色谱法/盐酸四环素/醋酸可的松/含量Key words
Cortisone tetracycline eye ointment/HPLC/Tetracycline hydrochloride/Cortisone acetate/Content分类
医药卫生引用本文复制引用
刘映倩,曾杰,邓贵福,李绪伦,伍彬..HPLC法同时测定四环素可的松眼膏中盐酸四环素和醋酸可的松的含量[J].中国药房,2015,(30):4274-4276,3.
