中国药房2015，Vol.26Issue(31)：4357-4359,3.DOI:10.6039/j.issn.1001-0408.2015.31.13
雷公藤甲素对类风湿关节炎患者滑膜成纤维样细胞增殖的体外抑制作用研究
Study on Inhibitory Effects of Triptolide on the Proliferation of Fibroblast-like Synovial Cells from Pa-tients with Rheumatoid Arthritis in vitro
摘要
Abstract
OBJECTIVE:To investigate the effects of triptolide(TP)on the proliferation of fibroblast-like synoviocytes(FLS) from patients with rheumatoid arthritis(RA)in vitro. METHODS:5 RA patients received knee arthroplasty or synovectomy to ob-tain synovial tissue. FLS was isolated,cultured and identified,and then incubated in the presence of TP [0 (blank control),50, 100 and 200 nmol/ml] for 24,48 and 72 h. The effects of TP on FLS was evaluated by MTT,and then proliferation inhibitory rate was calculated;flow cytometry was used to detect the apoptosis and cell cycle of FLS. RESULTS:The inhibitory rates of TP(50, 100 and 200 nmol/ml)on the proliferation of FLS were 17.46%-52.56%,which was positively correlated with drug concentration. Compared with blank control group,100 and 200 nmol/ml TP could increase the percentage of cells in G0/G1 phase and decrease the percentage of cells in S phase,with statistical significance(P<0.05);200 nmol/ml TP could induce cell apoptosis. CONCLU-SIONS:TP could inhibit the proliferation and also could induce the apoptosis of FLS in RA patients in vitro,which may be one of its mechanism for treating RA.关键词
类风湿关节炎/滑膜成纤维细胞/雷公藤甲素/增殖/抑制/机制Key words
Rheumatoid arthritis/Fibroblast-like synovial cells/Triptolide/Proliferation/Inhibitory/Mechanism分类
医药卫生引用本文复制引用
叶蕾,贾霜,潘文萍..雷公藤甲素对类风湿关节炎患者滑膜成纤维样细胞增殖的体外抑制作用研究[J].中国药房,2015,26(31):4357-4359,3.基金项目
山东省中医药科技发展计划项目(No.2011-191) （No.2011-191）
