中国药房2015，Vol.26Issue(31)：4405-4408,4.DOI:10.6039/j.issn.1001-0408.2015.31.28
大孔树脂纯化莲房总黄酮的工艺研究
Study on Purification Technology of Total Flavonoids from Nelumbinis receptaculum by Macroporous Resin
摘要
Abstract
OBJECTIVE:To study the purification technology of total flavonoids from Nelumbinis receptaculum by macropo-rous resin. METHODS:Using adsorption rate and desorption rate of total flavonoids from Nelumbinis receptaculum as index,the type of macroporous resin was selected by static adsorption-desorption tests;With adsorption rate of total flavonoids as index,sin-gle factor test was used to investigate the effects of the concentration of total flavonoids,adsorption time,adsorption speed, drug-loading amount,water amount,volume fraction and amount of eluant and other factors on the purification technology. The op-timal technology was validated. RESULTS:Among 10 kinds of resin,HPD-400 macroporous resin was found to have the best ad-sorption and desorption effects. The optimal purification conditions was as follows as the concentration of total flavonoids 7.00 mg/ml, adsorption time of 3 h,flow rate for sampling of 3 column volume (BV)/h,drug-loading amount of 8 BV,water amount of 6 BV,50% ethanol elution amount of 4 BV. In validation test,mass fraction of total flavonoids from purified Nelumbinis receptacu-lum were 63.88%,62.50% and 63.44%(RSD=1.11%,n=3). CONCLUSIONS:HPD-400 macroporous resin could purify total flavonoids from purified Nelumbinis receptaculum,and established purification technology is stable and practical.关键词
莲房/总黄酮/大孔树脂/纯化工艺Key words
Nelumbinis receptaculum/Total flavonoids/Macroporous resin/Purification technology分类
医药卫生引用本文复制引用
郑淑霞,易骏,吴锦忠,谭春江,吴建国,陈建忠,吴岩斌..大孔树脂纯化莲房总黄酮的工艺研究[J].中国药房,2015,26(31):4405-4408,4.基金项目
福建省科技厅社会发展重点项目(No.2013Y0058) （No.2013Y0058）
2013-2015年度福建省中医科研项目(No.wzzy 201301) （No.wzzy 201301）
