中国药房2015，Vol.26Issue(33)：4690-4692,3.DOI:10.6039/j.issn.1001-0408.2015.33.29
蒙药漏芦花的HPLC指纹图谱研究
Study on the HPLC Fingerprint Spectrum of Mongolian Medicine Rhaponticum uniflorum
摘要
Abstract
OBJECTIVE:To establish HPLC fingerprint sectrum of Rhaponticum uniflorum. METHODS:HPLC was performed on the column of Hypersil-ODS with mobile phase of 0.3% phosphoric acid-acetonitrile(gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 220 nm,column temperature was 30 ℃ and volume injection was 10 μl. The luteolin was refer-ence,17 batches of R. uniflorum from different production places was analyzed and similarity evaluation system for chromatograph-ic fingerprint of TCM (2004 A edition)was adopted for the similarity analysis. RESULTS:There were totally 11 common peaks with similarity degree≥0.900 of 17 batches. According to the verification,the fingerprint spectrum and reference fingerprint spec-trum of R. uniflorum had good consistency. CONCLUSIONS:The established method is specific and stable,and can provide refer-ence for the identification and quality control of R. uniflorum.关键词
蒙药/漏芦花/高效液相色谱法/指纹图谱Key words
Mongolian medicine/Rhaponticum uniflorum/HPLC/Fingerprint spectrum分类
医药卫生引用本文复制引用
田香,王美丽,白玉霞..蒙药漏芦花的HPLC指纹图谱研究[J].中国药房,2015,26(33):4690-4692,3.基金项目
国家科技支撑计划项目(No.2012BAI28B01) （No.2012BAI28B01）
内蒙古自治区高等学校科学研究项目(No.NJ10120) （No.NJ10120）
