中国药房2015，Vol.26Issue(33)：4734-4736,3.DOI:10.6039/j.issn.1001-0408.2015.33.45
HPLC法同时测定补乌酊中4种补骨脂类成分的含量
Simultaneous Determination of the Content of 4 Psoralen Compounds in Buwu Tincture by HPLC
夏天一 1陈顺 1高守红 1游春华 1张凤 1陆文铨 1朴淑娟 1陈万生1
作者信息
摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of 4 psoralen compounds in Buwu tincture. METHODS:HPLC was performed on the column of Dikma Diamonsil C18 with mobile phase of acetonitrile-0.2% Acetic acid by gradient elution at flow rate of 1 ml/min,detection wavelength was 246 nm,column temperature was 30 ℃,and the injection vol-ume was 15 μl. RESULTS:The linear range was 13.00-208 μg/ml for angelicin,26.00-416 μg/ml for bavachin,24.50-392 μg/ml for psoralidin and 37.88-606 μg/ml for isobavachalcone,respectively(r≥0.999 6);RSDs of precision,stability and reproducibility tests were less than 2.00%;recoveries were 95.22%-97.23%(RSD=0.87%,n=6),100.24%-104.64%(RSD=1.62%,n=6), 102.28%-104.39%(RSD=1.47%,n=6)and 97.68%-100.17%(RSD=0.97%,n=6),respectively. CONCLUSIONS:The method is simple and reproducible,and can be used for the quality control of Buwu tincture.关键词
高效液相色谱法/补乌酊/异补骨脂素/补骨脂甲素/补骨脂定/补骨脂乙素/含量Key words
HPLC/Buwu tincture/Angelicin/Bavachin/Psoralidin/Isobavachalcone/Content分类
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夏天一,陈顺,高守红,游春华,张凤,陆文铨,朴淑娟,陈万生..HPLC法同时测定补乌酊中4种补骨脂类成分的含量[J].中国药房,2015,26(33):4734-4736,3.
