摘要
Abstract
OBJECTIVE:To establish a method for the simultaneous determination of geniposide,paeoniflorin,berberine hy-drochloride,baicalin and paeonol in Qingwei huanglian pill. METHODS:HPLC was performed on the column of Diamonsil C18 with mobile phase of acetonitrile-0.3% phosphate(gradient elution)at flow rate of 1.0 ml/min,detection wavelength was 238 nm, column temperature was 30 ℃ and the injection volume was 10 μl. RESULTS:The linear range was 15.36-153.6 μg/ml for genipo-side, 6.56-65.6 μg/ml for paeoniflorin, 22.74-227.4 μg/ml for berberine hydrochloride, 26.06-260.6 μg/ml for baicalin and 5.57-55.7 μg/ml for paeonol(r≥0.999 8);RSDs of precision,stability and reproducibility tests were not higher than 1.0%;recov-eries were 96.28%-99.38%(RSD=1.1%,n=6),97.13%-99.48%(RSD=1.2%,n=6),98.14%-100.25(RSD=0.7%,n=6), 97.38%-100.05%(RSD=1.0%,n=6) and 96.30%-99.12%(RSD=1.2%,n=6),respectively. CONCLUSIONS:The method is simple and good reproducibility,and suitable for the contents determination of geniposide,paeoniflorin,berberine hydrochloride, baicalin and paeonol in Qingwei huanglian pill.关键词
高效液相色谱法/清胃黄连丸/栀子苷/芍药苷/盐酸小檗碱/黄芩苷/丹皮酚/含量测定Key words
HPLC/Qingwei huanglian pill/Geniposide/Paeoniflorin/Berberine hydrochloride/Baicalin/Paeonol/Content determination分类
医药卫生
