中国药房Issue(34)：4775-4778,4.DOI:10.6039/j.issn.1001-0408.2015.34.08
二氢丹参酮对人肺癌GLC-82细胞的抑制作用及其机制研究
Inhibitory Effect and Its Mechanism of Dihydrotanshinone on Human Lung Cancer GLC-82 Cell
摘要
Abstract
OBJECTIVE:To study the inhibitory effect of dihydrotanshinone(DTS)on human lung cancer GLC-82 cell and its mechanism. METHODS:After treated with 0(blank control),5,10,20,40,80 and 100 µg/ml DTS for 24 and 48 h,MTT as-say was used to measure the inhibition rates and IC50 of cells;cell apoptosis was detected by flow cytometry after treated with 17.85 µg/ml DTS for 12,24 and 48 h to calculate apoptotic rate;Western blot was used to detect the protein expressions of Bcl-2, Bax and Caspase-3. RESULTS:Compared with blank control group,different concentrations of DTS inhibited the proliferation of cells;24 and 48 h maximal inhibition rate were 54.48% and 64.95%,respectively;IC50 were 62.36 and 33.94 µg/ml. DTS could induce cell apoptosis in positive time dependent manner,and the range of inhibition rate was 5.6%-29.6%;Western blot showed DTS could down-regulate the expression of Bcl-2 protein and up-regulate the expression of Caspase-3 protein (P<0.01 or P<0.05). CONCLUSIONS:DTS have significant inhibitory effect on GLC-82 cells and also induce cell apoptosis,by a possible mech-anism of down-regulating the expression of Bcl-2 protein and up-regulating the expression of Caspase-3 protein.关键词
人肺癌GLC-82细胞/二氢丹参酮/细胞凋亡/半胱氨酸天冬氨酸蛋白激酶3蛋白Key words
Human lung cancer GLC-82 cell/Dihydrotanshinone/Cell apoptosis/Caspase-3 protein分类
医药卫生引用本文复制引用
孙蓓,叶因涛,王冬,钱钧强,娄建石..二氢丹参酮对人肺癌GLC-82细胞的抑制作用及其机制研究[J].中国药房,2015,(34):4775-4778,4.基金项目
天津医科大学科学基金青年项目 （）
