中国药房Issue(34)：4847-4849,4850,4.DOI:10.6039/j.issn.1001-0408.2015.34.31
响应面法优化巴戟天低聚糖提取工艺
Optimization of the Extraction Technology for Oligosaccharides of Morinda officinalis by Response Surface Methodology
杨欣 1宋健平 2关业枝 2王琪 2林励 1林伟栋 1顾善喜1
作者信息
- 1. 广州中医药大学中药学院，广州 510006
- 2. 广州中医药大学科技产业园，广州 510445
- 折叠
摘要
Abstract
OBJECTIVE:To optimize the extraction technology of oligosaccharides of Morinda officinalis. METHODS:The liq-uid/solid ratio,extraction time and temperature were chosen as factors,and the yield of oligosaccharides was estimated as index. On the basis of single-factor experiments,using a 3-factor,3-level Box-behnken central composite experimental design,two-poly-nomial regression equation of extraction rate of oligosaccharide was established,and it was analyzed by response surface methodolo-gy to obtain the optimum extraction conditions,and the verification test was conducted. RESULTS:The optimum extraction condi-tions were as follows as material/liquid ratio of 23∶1(ml/g),extraction time of 1.7 h,and extraction temperature of 93℃,extract-ing for twice. Under these conditions, the estimated and observed average values of extraction rate of oligosaccharides were 10.37% and 10.29%(RSD=0.20%,n=3),respectively. The deviation value was 0.06%. CONCLUSIONS:The response surface methodology can be used to optimize the extraction process of oligosaccharides of M. officinalis.关键词
巴戟天/低聚糖/提取工艺/响应面法/优化Key words
Morinda officinalis How/Oligosaccharides/Extraction technology/Response surface methodology/Optimization分类
医药卫生引用本文复制引用
杨欣,宋健平,关业枝,王琪,林励,林伟栋,顾善喜..响应面法优化巴戟天低聚糖提取工艺[J].中国药房,2015,(34):4847-4849,4850,4.
