中国医科大学学报2015，Vol.44Issue(9)：769-774,6.
MiR?429对人U87胶质瘤细胞增殖、迁移和侵袭能力的影响及其作用机制
The Effect and Mechanism of miR?429 Regulating Proliferation,Migration and Invasion of Human U87 Glioma Cells
摘要
Abstract
Objective To study the effects of miR?429 on proliferation,migration and invasion of human glioma U87 cells and the potential mecha?nism. Methods After human U87 glioma cells were cultured,Lipofectamine? LTX was applied to stably transfect U87 glioma cells with pre?miR?429 and anti?miR?429 plasmids(shRNA plasmid vector). After verification of the efficiency of transfection by real?time PCR,CCK?8 was applied to detect the change in proliferation of U87 cells. The Transwell chamber assay was applied to measure the change in migration and invasion of hu?man U87 glioma cells,the real?time PCR and the Western blot assay were applied to measure the change in mRNA and protein levels of zinc finger E?box binding homeobox 1(ZEB1). Normal U87 cells and miR?429?overexpressing U87 cells were transfected with ZEB1,and the Transwell chamber assay was applied to detect the effect of single overexpressed or co?overexpressed miR?429 and ZEB1 on proliferation,migration and invasion of human U87 glioma cells. Results Compared with the control group,overexpression of miR?429 significantly inhibited the proliferation,migration and invasion ability of U87 cells and obviously reduced the expression level of mRNA and protein of ZEB1 in human U87 glioma cells. Overexpres?sion of ZEB1 dramatically improved the proliferation,migration and invasion of human U87 glioma cells,furthermore,effectively blocked the func?tion of miR?429 overexpression inhibiting proliferation,migration and invasion. Conclusion MiR?429 reduces proliferation,migration and inva?sion of U87 glioma cells by inhibiting ZEB1.关键词
miR?429/E盒结合锌指蛋白1/人U87胶质瘤细胞/增殖/迁移/侵袭Key words
miR?429/zinc finger E?box binding homeobox 1/human U87 glioma cells/proliferation/migration/invasion分类
医药卫生引用本文复制引用
陈亮宇,刘丽波,李新星,喻博,洪杨,郑健,于奇,薛一雪,刘云会..MiR?429对人U87胶质瘤细胞增殖、迁移和侵袭能力的影响及其作用机制[J].中国医科大学学报,2015,44(9):769-774,6.基金项目
国家自然科学基金(81402573,81372484,81372682, 81272564,81172197,81171131) （81402573,81372484,81372682, 81272564,81172197,81171131）
