中国医科大学学报2015，Vol.44Issue(9)：796-798,802,4.
单纯疱疹病毒Ⅰ型的Th细胞和B细胞表位重组核酸疫苗的构建及真核表达
Construction and Eukaryotic Expression of HSV?1 Th and B Lymphocytes Recombinant Multi?epitope DNA Vaccine
摘要
Abstract
Objective To construct the recombinant multi?epitope DNA vaccine of HSV?1 Th and B lymphocytes of gB?gD protein,and to identify its eukaryotic expression. Methods The epitopes of HSV?1 gB?gD protein were analyzed by bioinformatics analysis software. HSV?1 Th and B lym?phocyte recombinant multi?epitope gene(Th/B gene)was designed and synthesized. Th/B gene was cloned into pVAX1 to synthesize pVAX1?Th/B. pVAX1?Th/B was then transfected into COS?7 cell,and the protein expression was identified by Western blot. Results The epitopes of Th and B lymphocytes of HSV?1 gB and gD were predicted,and the eukaryotic expression plasmid pf pVAX1?Th/B was successfully constructed and con?firmed by sequencing. In addition,the in vivo protein expression of the recombinant DNA was confirmed by Western blot. Conclusion HSV?1 Th and B lymphocyte recombinant multi?epitope DNA vaccine was successfully synthesized in this study. HSV?1 Th and B lymphocyte recombinant multi?epitope DNA vaccine can be successfully expressed in isolated eukaryotic cells. This study provides a feasible solution for developing DNA vac?cine against HSV?1 infection.关键词
单纯疱疹病毒/Th细胞和B细胞表位/核酸疫苗Key words
herpes simplex virus/Th cell and B cell epitope/DNA vaccine分类
医药卫生引用本文复制引用
刘贤洁,孙原,马小力..单纯疱疹病毒Ⅰ型的Th细胞和B细胞表位重组核酸疫苗的构建及真核表达[J].中国医科大学学报,2015,44(9):796-798,802,4.基金项目
国家自然科学基金(81200656) （81200656）
辽宁省博士启动基金(20111097) （20111097）
