中国药理学通报Issue(10)：1421-1425,1426,6.DOI:10.3969/j.issn.1001-1978.2015.10.019
三种化学小分子对补体旁路激活致内皮细胞黏附分子表达的干预及机制研究
Effect of three chemical molecules on adhesion molecules expression in HMECs induced by activated complement alternative pathway
摘要
Abstract
Aim To investigate the effect of resveratrol ( Res) , PDTC and AG490 on adhesion molecules ex-pression induced by product of activated complement alternative pathway on human microvascular endothelial cells ( HMECs) and the possible mechanisms. Meth-ods HMECs were exposed to the product of comple-ment alternative pathway activation, then the superna-tant was removed to detect the concentration of malond-ialdehyde ( MDA ) with TBA method. ELISA method was used to detect the expression of soluble ICAM-1 , VCAM-1 ( and E-selectin) in the culture supernatant. Res, PDTC and AG490 with different concentrations were used to determine their effect on cell oxidation level and adhesion molecules expression. The phospho-rylation of NF-κB p65 was detected by Western blot, and the intervention of Res, PDTC and AG490 was as-sayed by the same way. Results The activation of complements alternative pathway resulted in the phos-phorylation of NF-κB p65 , and increased the concen-tration of MDA and up-regulated the expression of ICAM-1, VCAM-1 and E-selectin. Res reduced the concentration of MDA. Res, PDTC and AG490 inhibi-ted the phosphorylation of NF-κB p65 . Res and PDTC showed similar inhibition on expression of ICAM-1 and VCAM-1 , while exhibiting little effect on expression of E-selectin, and AG490 significantly inhibited the ex-pression of the above adhesion molecules. Conclusions Res, PDTC and AG490 could inhibit the expression of adhesion molecules induced by activated complement alternative pathway, the inhibition of NF-κB pathway activation was involved in their mechanism, and JAK2 may be a more important intervention target in regula-ting adhesion molecule expression.关键词
补体/补体旁路激活/内皮细胞/黏附分子/白藜芦醇/炎症反应/眼镜蛇毒因子Key words
complement/complement alternative path-way ( activation )/endothelial cell/adhesion molecule/resveratrol/inflammation/cobra venom factor分类
医药卫生引用本文复制引用
李朝胜,孙黔云..三种化学小分子对补体旁路激活致内皮细胞黏附分子表达的干预及机制研究[J].中国药理学通报,2015,(10):1421-1425,1426,6.基金项目
国家自然科学基金资助项目( No 31060124) （ No 31060124）
贵州省优秀科技教育人才省长专项资金资助项目[黔省专合字(2012)9号] （2012）
