中国药理学通报Issue(10)：1426-1432,7.DOI:10.3969/j.issn.1001-1978.2015.10.020
异甘草素诱导人黑色素瘤A375细胞凋亡研究
Isoliquiritigenin induced apoptosis in human melanoma A375 cells
摘要
Abstract
Aim To evaluate the mechanism of apopto-sis induced by the isoliquiritigenin in A375 human ma-lignant melanoma cells. Methods Sulforhodamine B ( SRB) method was used to determine the A375 cell viability;acridine orange/ethidium bromide ( AO/EB) and Hoechst 33258 staining were used to observe the morphological changes of apoptotic cells; flow cytome-try was used to detect A375 cell apoptotic rate;DCFH-DA was applied to determine the changes of total intra-cellular ROS in A375 cells;JC-1 method was used to measure the changes of mitochondrial membrane poten-tial;the kits methods were used to determine the con-tent of ATP, lactic acid and glucose in A375 cell which was treated with different concentrations of isoliquiritigenin. Results Isoliquiritigenin could in-hibit A375 cell proliferation in a concentration-depend-ent manner; A375 cells showed obvious apoptosis charateristics after treatment by isoliquiritigenin, and the apoptosis rate increased with increasing concentra-tion of isoliquiritigenin. The level of total intracellular ROS in A375 cells increased obviously after dealing with different concentrations of isoliquiritigenin;in ad-dition, the mitochondrial membrane potential, the lev-els of intracellular ATP,lactic acid and the level of glu-cose uptake all declined. Conclusions These find-ings demonstrate that isoliquiritigenin can induce apop-tosis of A375 cells. The mechanism may be related to elevation of ROS level and reduction of aerobic glycoly-sis level.关键词
异甘草素/人黑色素瘤细胞/细胞凋亡/凋亡形态/活性氧/线粒体膜电位/糖酵解Key words
isoliquiritigenin/human melanoma cells/cell apoptosis/apoptotic morphology ROS/apoptotic morphology/mitochondrial membrane potential/gly-colysis分类
医药卫生引用本文复制引用
阎新燕,司玲玲,高彩霞,于丽娜,王艳明,郑秋生..异甘草素诱导人黑色素瘤A375细胞凋亡研究[J].中国药理学通报,2015,(10):1426-1432,7.基金项目
国家自然科学基金资助项目( No 31471338) （ No 31471338）
科技部科技型中小企业创业投资引导基金(No 13C26216516410)兵团重点领域创新团队资助项目(2015BD005) （No 13C26216516410）
