中国药理学通报Issue(11)：1569-1574,1575,7.DOI:10.3969/j.issn.1001-1978.2015.11.018
大黄素激活AMPK、PPARγ对3T3-L1脂肪细胞葡萄糖摄取的影响
Activation of AMPK and PPARγby emodin influences glucose uptake in 3 T3-L1 adipocytes
摘要
Abstract
Aim To investigate glucose uptake effects and mechanism of emodin in 3T3-L1 adipocytes. Methods LPS-induced differentiated 3 T3-L1 adipo-cytes were divided into control group and emodin ( 1 , 10, 50 μmol · L-1 ) groups. Then, 6-NBDG uptake and the expression of cell surface GLUT4 , PPARγ, AMPKα1/2 , p-AMPKα1/2 , IRS-1 , p-IRS-1 , Adi-ponectin, chREBP-α and chREBP-β were detected. The ability of 6-NBDG uptake in LPS-induced 3 T3-L1 adipocytes was also evaluated following interference with AMPK inhibitor and PPARγinhibitor, respective-ly. Meanwhile, STZ-induced diabetic rats were ran-domly divided into control group and emodin treatment group. The mRNA expression of Adiponectin and pro-tein expression of cell surface GLUT4 , AMPKα1/2 , p-AMPKα1/2 were measured. Results Compared with the control group, emodin improved the mRNA expres-sion of cell surface GLUT4, Adiponectin, chREBP-αand chREBP-β, and protein expression of cell surface GLUT4 , PPARγ, IRS-1 , p-IRS-1 , AMPKα1/2 and p-AMPKα1/2 in 3T3-L1 adipocytes(P<0. 05). Emodin enhanced 6-NBDG uptake and the uptake of emodin group was both decreased following interference with AMPK inhibitor and PPARγ inhibitor, respectively ( P<0. 05 ) . Emodin also increased the mRNA expression of Adiponectin and protein expression of cell surface GLUT4 , AMPKα1/2 and p-AMPKα1/2 in adipose tis-sue of T2 DM rats ( P <0. 05 ) . Conclusion Emodin can enhance glucose uptake in 3 T3-L1 adipocytes and the mechanism is probably associated with activating Adiponectin and IRS-1 , thereby activating AMPK and PPARγ.关键词
大黄素/2型糖尿病/葡萄糖摄取/3 T3-L1细胞/葡萄糖转运体4/AMPK/PPARγKey words
emodin/type 2 diabetes mellitus/glucose uptake/3 T3-L1 cells/glucose transporter type 4/AMPK/PPARγ分类
医药卫生引用本文复制引用
洪海棉,谢秀利,洪桂祝,赖文芳..大黄素激活AMPK、PPARγ对3T3-L1脂肪细胞葡萄糖摄取的影响[J].中国药理学通报,2015,(11):1569-1574,1575,7.基金项目
福建省自然科学基金资助项目(No 2015J01328) （No 2015J01328）
福建中医药大学校管课题( No X2015013-平台) （ No X2015013-平台）
