中国医学创新Issue(1)：1-2,3,3.DOI:10.3969/j.issn.1674-4985.2013.01.001
改良血管阻塞方法建立大鼠全脑缺血模型
Modified Vascular Occlusion to Establish Cerebral Global Ischemia Model
摘要
Abstract
Objective:To explore the feasibility of modified vertebral artery occlusion to establish global cerebral ischemia rat model.Method:48 adult healthy male wistar rats were randomly divided into three groups: the sham operation group(group S,n=8),the traditional group(group T, n=30)and the improved group(group I,n=30).The rats from group T were done by three steps.First,we enlarged the alar foramina of the first cervical vertebra through which the vertebal arteries travelled with the grinding drill in order to visualize the vertebral arterises sufficiently and then coagulated the vertebral arteries by the microscope.Second,bilateral common cervical artery were dissociated and threaded. Finally,the bilateral common cervical artery were clamped for 10 minutes after 24 hours.The rats from group I accepted the same procedure as rats from group T except the bialteral vertebral arteries were interdicted with the electric iron which made by ourself.The rats from group S accepted the same procedure as the rats from group T except that the vertebral arteries were not interdicted and the bialteral vertebral arteries were not clamped. Both model groups were assessed with fees、the ratio of success and preparation time and costs.The group S was compared with the group I and T in ethology and pathology.Result:Compared with group T,the modified surgical approach can increase the successful ratio and reduce preparation time and costs(P<0.05).HE and Morris showed that group T and I had significant difference with group S in pathology and behavior(P<0.01).Conclusion:The group T and I were successful,but cerebral ischemia model with modified artery occlusion is more simple and reliable.关键词
全脑缺血模型/四血管阻塞法/椎动脉Key words
Global brain ischemic model/Four-vessel occlusion/Vertebral artery引用本文复制引用
严智文,董河,冯伟,牛泽军,褚海辰,隋爱华..改良血管阻塞方法建立大鼠全脑缺血模型[J].中国医学创新,2013,(1):1-2,3,3.基金项目
山东省自然科学基金(编号:Y2006C14) （编号:Y2006C14）
