变性高效液相色谱法检测晚期肺腺癌人类表皮生长因子受体2突变的探索性研究OA
Exploratory Study of Detecting Human Epidermal Growth Factor Receptor-2 Mutation Using Denaturing High Performance Liquid Chromatography in Advanced Lung Adenocarcinoma
目的：探索性研究变性高效液相色谱法在检测晚期肺腺癌人类表皮生长因子受体2突变中的应用价值。方法：应用变性高效液相色谱法(denaturing high-performance liquid chromatography,DHPLC)检测了52例晚期肺腺癌患者的血液样本提取的 DNA 中 HER2基因20号外显子的体细胞非移码插入突变,并结合直接测序进行了验证。结果：在对52例晚期肺腺癌患者血清标本提取的 DNA 检测中,通过野生型样本和未知样…查看全部>>
Objective:To exploratory research denaturing high performance liquid chromatography in detection of advanced lung adenocarcinoma human epidermal growth factor receptor 2 mutations in the application.Method:DHPLC was used to detect somatic HER2 Exon 20 somatic non-frameshifting mutations from 52 advanced lung adenocarcinoma patient serum samples.Result:Screened 52 samples with a mixture of 1:5 wild-type and unknown samples and found 3 HER2 Exon 20 YVMA insers…查看全部>>
赵莹莹;卢绍禹;杜文;梁洁;杨艳华
天津市第四医院 天津 300222天津市第四医院 天津 300222天津市第四医院 天津 300222天津市第四医院 天津 300222天津市第四医院 天津 300222
肺癌腺癌人类表皮生长因子受体2突变
Lung cancerAdenocarcinomaHuman epidermal growth factor receptor-2Mutation
《中国医学创新》 2013 (7)
1-2,3,3
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