中国医学创新Issue(10)：8-9,2.DOI:10.3969/j.issn.1674-4985.2013.10.004
内源性神经激肽和降钙素基因相关肽在大鼠心肌缺血后适应对心肌超氧化物歧化酶的作用
The Role of Endogenous Neurokinin and CGRP to Myocardial Superoxide Dismutase of Ischemic Postconditioning in Rats
摘要
Abstract
Objective:To investigate the role of endogenous neurokinin and CGRP in modulation of myocardial superoxide dismutase in ischemic postconditioning in rats.Method:Adult male SD rats(weighting 350-400 g)were randomly divided into 5 groups:sham group(only threading without ligation of coronary artery),I/R group(ligation of coronary artery for 30 minutes,then reperfusion for 120 minutes),ipost group(ligation of coronary artery for 30 minutes,then repeated three cycles of release of the coronary artery for 10 seconds and 10 seconds of ligation of coronary artery before reperfusion of the heart for 120 minutes),CGRP8-37+ipost group(CGRP8-37,a selective antagonist of CGRP receptor,was given(1μg/kg,i.v.,) through caudal vein at 5 minutes before the ischemic postconditioning),D-SP+ipost group(D-SP,a selective antagonist of neurokinin-1 receptor was injected(1μg/kg)via caudal vein at 5 minutes before the repeated three times of perfusion.The samples of myocardium was collected and frozen iliquid nitrogen and prepared for quantitative analysis. Detection the expression of SOD used Elisa,detection the expression of Caspase-3 used spectrophotometry. Result:(1)Compared with sham group,The expression of Caspase-3 and SOD in I/R group were increased(P<0.01).(2)Compared with I/R group, The expression of SOD in ipost group was increased(P>0.05),The expression of Caspase-3 in ipost group was decreased(P<0.01).(3)Compared with ipost group,The expression of SOD was decreased(P<0.01)in CGRP8-37+ipost group and D-SP+ipost group.The expresson of Caspase-3 was increased(P>0.05)in CGRP8-37+ipost group,D-SP+ipost group,but the difference did not show statistical significance.Conclusion:The results of this study indicate that endogenous neurokinin and CGRP participate in the anti-apoptosis through up-rewgulation of the expression of myocardial superoxide dismutase in ischemic post-conditioning in rats.关键词
NK1受体/CGRP受体/缺血后适应/超氧化物歧化酶/Caspase-3Key words
NK1 receptor/CGRP receptor/Ischemic postconditioning/SOD/Caspase-3引用本文复制引用
贾晓鹰,郭政,郭建丽,刘亚兵..内源性神经激肽和降钙素基因相关肽在大鼠心肌缺血后适应对心肌超氧化物歧化酶的作用[J].中国医学创新,2013,(10):8-9,2.基金项目
国家自然科学基金(编号:30972860) （编号:30972860）
