中国医学创新Issue(16)：6-8,3.DOI:10.3969/j.issn.1674-4985.2013.16.003
PTK787对K562细胞增殖及细胞周期作用的影响*
The Effects of PTK787 on K562 Cell Proliferation and Cell Cycle
摘要
Abstract
Objective:To investigate the effects of tyrosine kinase inhibitors PTK787 on cell proliferation and cell cycle of human chronic myeloid leukemia (CML)cell line K562,and to explore the mechanism of PTK787 against acute myeloid leukemia.Method:The MTT method was used to detect the effects of PTK787 in various concentrations and at different time points on proliferation of K562 cells,the flow cytometry was used to determine the effects of PTK787 in different time points on proliferation of K562 cells.Result:The result showed that along with increasing of the concentration and prolonging of time,the inhibitory rate of PTK787 on K562 proliferation was gradually enhanced.The comparison between various concentration groups at same time or comparison between various time groups in same concentration showed significant differences(P<0.05),in which the effect of 320 μmol/L PTK787 on cells was strongest while the continuous increase of PTK787 concentration or prolong of action time did not enhance the inhibitory rate on K562 proliferation.With increasing of drug concentration,the cell proportion in G1 phase gradually increased,the cell proportion in S phase gradually decreased,the comparison between various groups revealed significant differences (p<0.05),however the continuous increase of drug concentration from 160 μmol/L did not obviously change the cell proportion in phases of cell cycle.Conclusion:PTK787 shows effect of anti-leukemia in cells through inhibiting transformation of the K562 cells from G1 phase in to S phase.关键词
酪氨酸激酶抑制剂/白血病/K562细胞/PTK787/细胞增殖Key words
PTKs/Leukemia/K562 cell/PTK787/Cell proliferation引用本文复制引用
姜杉,陈日玲,邸晓华,刘晓利,田川..PTK787对K562细胞增殖及细胞周期作用的影响*[J].中国医学创新,2013,(16):6-8,3.基金项目
广东省卫生厅项目资助 （）
