中国医学创新2015，Vol.12Issue(30)：116-118,3.DOI:10.3969/j.issn.1674-4985.2015.30.038
三氧化二砷对食管癌细胞株EC-1的生长抑制作用
Effets of Aresenic Trioxide on Esophageal Squamous Carcinoma Cell Line EC-1
摘要
Abstract
Objective:To investigate effects of arsenic trioxide (As2O3) on esophageal squamous carcinoma cell line EC-1.Method:The inhibition effect of As2O3(different doses of 0.5,1,2,4,8,16,32,64 μmol/L) on EC-1 cells proliferation at different time points(24,48,72 h) was detected by MTT assay,and the value of IC50 was calculated .Cell cycle change and cell apoptosis were detected by flow cytometric analysis (FCM).Result:As2O3 was able to inhibit the proliferation of esophageal squamous carcinoma cell line EC-1,with the dose of As2O3 increasing and the effect time prolonging,the inhibition of cells proliferation was enhanced.The IC50 in EC-1 cells was 18.74 μmol/L. As2O3 could induced G2/M phase arrest and the reduction of the ratio of cells in G0/G1 and S phases expectively. As2O3 could enhance the radiation-induced apoptosis.Conclusion:The proliefration inhibition action of As2O3 on esophageal cell line Ec-1 depends on the concentration and the contact time. As2O3 hyperthermia can enhance the killing effect of radiation on tumour cells mainly through affecting the EC-l cell cycle, and the induction of apoptosis is the second reason.关键词
食管癌EC-1细胞系/三氧化二砷Key words
Human esophageal carcinoma EC-1 cell line/Arsenic trioxide引用本文复制引用
谷见法,王晓敏,路平..三氧化二砷对食管癌细胞株EC-1的生长抑制作用[J].中国医学创新,2015,12(30):116-118,3.基金项目
河南省科技厅资助项目(082300450160) （082300450160）
