中国肿瘤外科杂志Issue(5)：282-286,5.DOI:10.3969/j.issn.1674-4136.2015.05.003
miR-17-5 P对肺癌细胞株A549、SPCA-1和GLC-82的增殖及侵袭能力的影响
The impose of miR-17-5P over cell proliferation and invasion in lung cancer cells A549, SPCA-1 and GLC-82
摘要
Abstract
Objective The aim of this study was to investigate the effect and molecular mechanism of miR-17-5P over cell proliferation and invasion in lung cancer cells A549 , SPCA-1 and GLC-82. Methods miR-17-5P mimics and inhibitor were synthesized and transfected into lung cancer cells A549, SPCA-1 and GLC-82. The abilities of cell growth,migration and invasion were examined by MTT,Transwell, and Boyden chamber. Westen blot was used to test the expression of cell cycle and EMT-associated proteins. Results Compared with the control group,over expression of miR-17-5P improved A549, SPCA-1 and GLC-82 cells growth and number across polycarbonate membrane(P <0. 001). While miR-17-5P expression was inhibited, cells growth and number across polycarbonate membrane were improved(P<0. 001). Over expression of miR-17-5P upregulate the expression of CCND1,Vimentin and N-CA in A549, SPCA-1 and GLC-82. Meantime, expression of CC-ND1,Vimentin and N-CA was downgraded when expression of miR-17-5P was inhibited. Conclusion miR-17-5P may facilitate cell proliferation, migration and invasion in A549 , SPCA-1 and GLC-82, by which mechanism is related to the improvement of expression of CCNDl,Vimentin and N-CA.关键词
miR-17-5P/肺癌/增殖/侵袭Key words
miR-17-5P/lung cancer/proliferation/invasion引用本文复制引用
孟丽娟,王峻,樊卫飞,蒲骁麟,许菊青,王琳,杨民,刘福银..miR-17-5 P对肺癌细胞株A549、SPCA-1和GLC-82的增殖及侵袭能力的影响[J].中国肿瘤外科杂志,2015,(5):282-286,5.基金项目
江苏省基础研究计划(BK2011842) （BK2011842）
江苏省“六大人才高峰”项目(2012-WSN-045) （2012-WSN-045）
