中国癌症防治杂志Issue(1)：1-5,5.DOI:10.3969/j.issn.1674-5671.2013.01.01
淋巴管内皮细胞微环境对卵巢癌淋巴结定向高转移细胞上皮-间质转化的影响
Ability of lymphatic endothelial cell microenvironment to affect the epithelial-mesenchymal transition in ovarian carcinoma cell lines showing highly lymphatic directional metastasis
摘要
Abstract
Objective To explore whether creating a human lymphatic endothelial cell microenvironment under culture or co-culture conditions can affect the invasion and migration activity and the epithelial-mesenchymal transition in the ovarian carcinoma SKOV3-PM4 cell line,which shows highly lymphatic directional metastasis. Methods SKOV3-PM4 cells were cultured in a lymphatic endothelial cell microenvironment either by using conditioned medium from cultures of human lens epithelium cells(HLECs)or by co-culturing with HLECs.The ability of SKOV3-PM4 cells to invade and migrate was observed using transwell chambers.Expression of transforming growth factor (TGF-β1),E-cadherin and vimentin in SKOV3-PM4 cells was analyzed by immunohistochemistry. Results The proportion of SKOV3-PM4 cultures showing invasion after incubation in HLEC-conditioned medium was(53±11)%,compared to (30±5)% for control cells;the proportion of SKOV3-PM4 cultures showing migration behavior was(48±5)%,compared to(34±2)% for control cells.Expression of TGF-β1 and vimentin was up-regulated and expression of E-cadherin down-regulated in SKOV3-PM4 cells after culturing them in HLEC-conditioned medium or co-culturing them with HLECs. Conclusions A human lymphatic endothelial cell microenvironment can alter the epithelial-mesenchymal transition in ovarian carcinoma cells and enhance their invasion and migration ability.关键词
卵巢肿瘤/卵巢癌淋巴结定向高转移/人淋巴管内皮细胞/上皮-间质转化/微环境Key words
Ovarian neoplasms/Ovarian carcinoma line with highly lymphatic directional metastasis/Human lymphatic endothelial cells/Epithelial-mesenchymal transition/Microenvironment分类
医药卫生引用本文复制引用
刘丽,黎丹戎,钟艳平,李力,高婷,阮和云,冠潇..淋巴管内皮细胞微环境对卵巢癌淋巴结定向高转移细胞上皮-间质转化的影响[J].中国癌症防治杂志,2013,(1):1-5,5.基金项目
国家自然科学基金资助项目（81060218）；教育部高等学校博士学科点专项科研基金资助项目（20050598002）；广西自然科学基金资助项目 （）
