中国癌症防治杂志Issue(2)：113-116,4.DOI:10.3969/j.issn.1674-5671.2013.02.07
SATB1基因克隆及pEGFP-SATB1真核表达载体的构建和鉴定
Cloning of SATB1 cDNA and subcloning into a eukaryotic expression vector
摘要
Abstract
Objective To clone the cDNA of the gene encoding special AT-rich sequence binding protein(SATB1)and to subclone it into eukaryotic expression vector pEGFP in order to investigate the function and mechanism of action of SATB1. Methods SATB1 cDNA was cloned from pCMV6-XL6-SATB1 by polymerase chain reaction(PCR)and then the target gene was subcloned into pGEM-T and subsequently into the eukaryotic expression vector pEGFP-N1 to give pEGFP-SATB1. Results The complete cDNA sequence of SATB1(2312 bp)was consistent with the reported sequence(Genbank BC001744.1).Sequencing and restriction digest analysis re-vealed the target gene to be inserted in the expression vector in the correct orientation. Conclusion The SATB1 cDNA was success-fully cloned and used to construct recombinant eukaryotic expression vector pEGFP-SATB1.关键词
特异AT序列结合蛋白1/基因克隆/真核表达载体Key words
SATB1 gene/Gene cloning/Eukaryotic expression vector分类
医药卫生引用本文复制引用
..SATB1基因克隆及pEGFP-SATB1真核表达载体的构建和鉴定[J].中国癌症防治杂志,2013,(2):113-116,4.基金项目
广西自然科学基金资助项目﹙桂科自0991230﹚ （）
