摘要
Abstract
Objective To explore the mechanism of metformin in inhibiting breast cancer cell line MCF-7. Methods MCF-7 cells were cultured for 48 h in the presence or absence of 16.56 g/L metformin,total protein was extracted from cells and expression of nu-clear factor-kappa B(NF-kB)p65 and Lin28B were detected by Western blot. Furthermore,transcription levels of HMGA2 and H-Ras, which are regulated by microRNA let-7,were determined by RT-PCR. Results NF-kB p65 and Lin28B expression declined signifi-cantly after 48-h drug treatment(t=23.44,t=14.24;P<0.05).Transcription levels of HMGA2 and H-Ras,which are related to self-renewal and multipotent differentiation of tumor cells,decreased 85%(P≥0.05)and 96%(P≥0.05),respectively,based on 2-△△Ct measurements. Conclusions Metformin at 16.56 g/L can effectively reduce NF-kB and Lin28B expression and inhibit HMGA2 and H-Ras transcrip-tion in MCF-7 cells.These findings suggest that metformin inhibits breast cancer at the cellular level.关键词
乳腺肿瘤/二甲双胍/NF-kB p65/Lin28B/HMGA2/H-RasKey words
Breast neoplasms/Metformin/NF-kB p65/Lin28B/HMGA2/H-Ras分类
医药卫生
