中国药业2015，Vol.24Issue(21)：15-17,3.
阿司匹林抑制去势大鼠DL-1,DL-6,TNF-α和M-CSF的表达及其防治绝经后骨质疏松作用的研究
Aspirin in Restraining IL-1, IL-6, TNF-α and M-CSF EXpression and Its Effect in PreVenting Post-Menopause Osteoporosis
摘要
Abstract
Objective To investigate the influence of aspirin on ovariectomized osteoporosis in mice interleukin-1 ( IL-1 ) , interleukin-6 ( IL-6 ) , tumor necrosis factor ( TNF-α) and macrophage colony-stimulating factor ( M-CSF ) expression and its role in prevention and treatment of osteoporosis. Methods 60 Sprague-Dawley rats were randomly divided into sham operation group ( control group, 10 rats ) and ovariectomized model group ( 50 rats ) , and the ovariectomized model group were then randomly divided into the osteoporosis group, group A ( given 0. 25 mmol/L aspirin ) , group B ( given 0. 5 mmol/L aspirin ) , group C ( given 1. 0 mmol/L aspirin ) and group D ( given 1. 5 mmol/L aspirin ) at 1 week after operation, 10 rats in each group. The serum and bone tissue IL-1, IL-6, TNF-α and M-CSF level were detected, while the scanning electron microscope was used to analyze every area of bone resorption. Results The IL-6, serum IL-1, TNF-α and M-CSF of the osteoporosis group were ( 80. 15 ± 7. 18 ) pg/mL, ( 82. 16 ± 6. 63 ) pg/mL, ( 30. 21 ± 4. 73 ) pg/mL and ( 11. 42 ± 4. 25 ) pg/mL respectively, which were significantly higher than those of the control group ( P < 0. 05 );in group A, B, C, D, the IL-1, IL-6, TNF-α and M-CSF levels of group D were the lowest ( P < 0. 05 );the bone tissue IL-1, IL-6, TNF-α and M-CSF of the osteoporosis group were ( 71. 24 ± 8. 10 ) pg/mL, ( 78. 02 ± 5. 15 ) pg/mL, ( 26. 92 ± 3. 23 ) pg/mL and ( 10. 22 ± 3. 15 ) pg/mL respectively, which were significantly higher than those of the control group ( P < 0. 05 );in group A, B, C, D, the IL-1, IL-6, TNF-α and M-CSF levels of group D were the lowest ( P < 0. 05 );the micro -CT analysis showed that the vertebral trabecular thickness, trabecular bone volume and bone mineral density of the aspirin group were significantly higher than those of the OVX group. Conclusion Aspirin can inhibit the serum and bone tissue of ovariectomized osteoporosis mice model IL-1, IL-6, TNF-α and M-CSF expression, weaken the osteoclast activity, and to prevent osteoporosis.关键词
阿司匹林/细胞因子/骨质疏松/大鼠Key words
aspirin/cell factor/osteoporosis/rat分类
医药卫生引用本文复制引用
杨超,曾艳平,杨桐,覃君慧,李海勋,桑宏勋..阿司匹林抑制去势大鼠DL-1,DL-6,TNF-α和M-CSF的表达及其防治绝经后骨质疏松作用的研究[J].中国药业,2015,24(21):15-17,3.基金项目
国家自然科学基金面上项目,项目编号:81270959. （）
