中国药业2015，Vol.24Issue(21)：103-105,3.
P53介导的活性氧通路对硒酸酯多糖诱导骨肉瘤细胞凋亡的作用
Role of P53 Mediated ROS Pathway in kappa-Selenocarrageenan Induced Osteosarcoma Tumor Cell ASpoptosis
来小丹 1冉启文 1欧阳净 1石英1
作者信息
- 1. 中国人民解放军第三军医大学第一附属医院药剂科,重庆 400000
- 折叠
摘要
Abstract
Objective To analyze the role of P53 mediated ROS pathway in Kappa-selenocarrageenan induced osteosarcoma tumor cell apoptosis. Methods Different concentrations selenocarrageenan were used to treat the OS-732 cells, the expression levels of pro-liferating cells, apoptosis, ROS level and apoptosis-related proteins in each group were analyzed. Results After selenocarrageenan pro-cessing, the OS-732 cell growth inhibition showed varying degrees with a time-and concentration-dependent manner, and 60 g/L concentration for 48 h was the maximum inhibition rate of OS-732;20, 40, 60 μg/mL after concentrations 24 h, ROS levels were sig-nificantly increased ( P < 0. 05 ) , OS-732 cell apoptosis was significantly increased, and the percentage of early apoptotic cells and late apoptotic cells were significantly different compared with 0 μg/mL ( P < 0. 05 );P53, Caspase-9 Bax expression increased with the in-crease of drug concentration, while the expression of Bcl-2 was significantly lowered ( P < 0. 05 ) . Conclusion Kappa-selenocar-rageenan can significantly inhibit OS-732 cell proliferation, induce apoptosis, and P53-mediated ROS pathway may play an important role.关键词
硒酸酯多糖/骨肉瘤/细胞凋亡Key words
kappa-selenocarrageenan/osteosarcoma/cell apoptosis分类
医药卫生引用本文复制引用
来小丹,冉启文,欧阳净,石英..P53介导的活性氧通路对硒酸酯多糖诱导骨肉瘤细胞凋亡的作用[J].中国药业,2015,24(21):103-105,3.
