中国药业2015，Vol.24Issue(21)：115-117,3.
高效液相色谱法波长切换法同时测定吴茱萸中3组分含量
Simultaneous Determination of 3 Components in Euodiae Fructus by HPLC WaVelength Switching Technology
摘要
Abstract
Objective To develop an HPLC method for simultaneous determination of 3 components in Euodiae Fructus. Methods A C18 column ( 250 mm × 4. 6 mm, 5 μm ) was used with the mobile phase of acetonitrile-0. 2% phosphoric acid ( 35:65 ) with the flow rate of 2. 0 mL/min and the column temperature was 30 ℃. The detection wavelength was set at 205 nm for Evodine in the first 15. 01 min, and then changed to 225 nm for evodiamine and rutaecarpine between 15. 01 and 28 min. Results The linear ranges of evodine, evo-diamine and rutaecarpine fell within the ranges of 0. 186~3. 72 μg, 0. 040 24~0. 804 8 μg, 0. 040 08~0. 801 6 μg, respectively ( r=0. 999 7,0. 999 9,0. 999 9 ) . The average recoveries were 96. 30%, 97. 66% and 97. 46% with RSD were 1. 43%, 1. 32% and 1. 80%, respectively( n=6). Conclusion The method is convenient,quick,accurate and suitable for the qulity control of Euodiae Fructus.关键词
吴茱萸/吴茱萸内酯/吴茱萸碱/吴茱萸次碱/高效液相色谱法/含量测定Key words
Euodiae Fructus/Evodine/Evodiamine/Rutaecarpine/HPLC/wavelength switching/determination分类
医药卫生引用本文复制引用
申丽莎,陈国庆,杨帆,邓开英..高效液相色谱法波长切换法同时测定吴茱萸中3组分含量[J].中国药业,2015,24(21):115-117,3.基金项目
重庆市科委自然科学基金计划资助项目,项目编号:cstc2012jjA10154. （）
