中国医药科学Issue(11)：27-29,3.
胡黄连苷Ⅱ对大鼠脑缺血损伤后NO含量和GSHPx活性的影响
The effect of picrosideⅡ on the concentration of NO and the activity of GSHPx after cerebral ischemic injury in rats
摘要
Abstract
Objective To optimize the therapeutic dose and time window of picrosede Ⅱ by orthogonal test in cerebral ischemic injury in rats. Methods The forebrain ischemia models were established by bilateral common carotid artery occlusion (BCCAO) methods. The successful models were randomly grouped according to orthogonal experimental design and treated by injecting picroside Ⅱ intraperitonenally at different ischemic time with different dose. The concentration nitric oxide (NO) in serum and brain tissue were determined by nitratase reduase assay. The activity of glutathione peroxidase (GSHPx) in serum and tissue were determined by chemical colormetry. Results The optimized composition of the therapeutic dose and time window of picrosideⅡin cerebral ischemic injury was (1) ischemia 1.5 h with 20 mg/kg body weight according to the concentrations of NO in serum and brain tissue;(2) ischemia 1.5 h with 10 mg/kg and ischemia 2.0 h with 20 mg/kg body weight according to the activities of GSHPx in serum and brain tissue. Conclusion From the principle of lowest therapeutic dose with longest tome window,the optimized composition of the therapeutic dose and time window of picrosideⅡin cerebral ischemic injury is ischemia 1.5-2.0 h with 10-20 mg/kg body weight.关键词
胡黄连苷Ⅱ/脑缺血/剂量/时间窗/NO/GSHPX/大鼠Key words
PicrosideⅡ/Cerebral ischemia/Therapeutic dose/Time window/NO/GSHPx/Rats分类
医药卫生引用本文复制引用
钟亮,张睿,纪晓军,吴艺玲,徐琦,刘洪玲,黄惠..胡黄连苷Ⅱ对大鼠脑缺血损伤后NO含量和GSHPx活性的影响[J].中国医药科学,2013,(11):27-29,3.基金项目
国家自然科学基金项目（81041092）；山东省自然科学基金项目（ZR2011HM050）。 （）
