摘要
Abstract
Objective Establish the HPLC method to determine the contents of rhynchophylline and isorhynchophylline in Uncaria rhynchophylla and provide scientific guidance for determining the contents of active ingredient in Uncaria rhynchophylla. Methods Contents of rhynchophylline and isorhynchophylline was measured by HPLC. Column of Diamonsi (250 nm×4.0 nm,5μm),solvent system:Methanol ︰ H2O(55 ︰ 45),UV wave:254 nm,Flow rate:1.0 mL/min,column temperature:28 ℃ ,Injection volume:20μL. Results We established the regression equation of rhynchophylline and isorhynchophylline Y=0.999 E+6x-21133,R2=0.9998 and Y=1.008 E+ 6x-13556,R2=0.9999,by making peak area as the ordinate and concentration as abscissa. Moreover this method got good results in precision,stability and repeatability. Conclusion The method that determining the contents of rhynchophylline and isorhynchophylline in Uncaria rhynchophylla by HPLC has the advantage on quick,simple,reproducible,and has a huge value in determining the contents of Chinese traditional Medicine.关键词
钩藤/钩藤碱/异钩藤碱/含量检测/高效液相色谱法Key words
Uncaria rhynchophylla/Rhynchophylline/Isorhynchophylline/Content determine/HPLC分类
医药卫生
