摘要
Abstract
OBJECTIVE:The HPLC method was adopted to determined the content of scopoletin and gallic acid, the method for the quality control of Fuke'an pills was developed, so as to control the product quality.METHODS:The thin-layer of Jasminum elongatum, Plantago asiatica L., Pericarpium granati and Chinese knotweed herb were determined by TLC.The scopoletin used C18 column, acetonitrile-water-ice-acetid acid (V∶V∶V=13∶87∶1) was set as mobile phase, and the wavelength of detector was set at 345 nm.The gallic acid used C18 column, methanol-0.5%ice -acetid acid ( V∶V=7∶93) was set as mobile phase, and the wavelength of detector was set at 270 nm. RESULTS:Scopoletin and gallic acid had been separated well in the thin-layer identification.When the line range of scopoletin was 3.44 μg/ml~41.28 μg/ml (r=0.999 9,n=5), the average recovery was [(100.6 ±1.07)%(n=9)].When the line range of gallic acid was 10.52μg/ml~63.12μg/m (r=0.999 93,n=5), the average recovery was [ (99.73 ±0.52%) ( n=9 ) ] .CONCLUSIONS: The method is simple, accurate, reliable with good specificity and reproducibility, it can be used as the quality standard of Fuke'an pills.关键词
腹可安片/质量标准/研究/东莨菪内酯/没食子酸Key words
Fuke'an pills/Quality standard/Studies/Scopoletin/Gallic acid分类
医药卫生
