中国真菌学杂志2015，Vol.10Issue(5)：307-311,5.
多重连接探针扩增技术在临床病原体检测中的应用
MLPA application in clinical diagnosis of pathogen
摘要
Abstract
As for patients diagnosed with Infectious diseases,favorable prognosis is determined by correct diagnosis and proper treatment.So it is important to diagnose clinical pathogens rapidly.Compared with other clinical test methods,MLPA is a simple,val?id,and fast one which can detect several gene sequences in quantity at the same time. The steps include:the degeneration of DNA sample,hybridization,ligation,and PCR amplification.Although being at its first stage,there are already certain applications of MLPA in diagnosing and researching of clinic microorganism,and the peculiarity of easy operation,fast reaction,high pass rate and high sen?sitivity have reveal bright prospect of MLPA.关键词
多重连接探针扩增/病原体/诊断Key words
multiplex ligation?dependent probe amplification/pathogen/diagnosis分类
医药卫生引用本文复制引用
方文捷,刘加,潘炜华,廖万清..多重连接探针扩增技术在临床病原体检测中的应用[J].中国真菌学杂志,2015,10(5):307-311,5.基金项目
国家973项目 (2013CB531601),重大传染病防治科技重大专项 (2013ZX10004612),上海市科委专项 (14DZ2272900) （2013CB531601）
