中国中西医结合外科杂志Issue(1)：40-42,3.DOI:10.3969/j.issn.1007-6948.2013.01.013
消栓通脉颗粒药物血清对TNF-α诱导人脐静脉内皮细胞NF-κB表达的影响
TNF-α Inducting Expression of Human Umbilical Vein Endothelial Cells NF-κB and Intervention of Chinese Herbal Medicine
摘要
Abstract
objective To research the expression of nuclear transcription factors NF-κB in the TNF-α in⁃duction of human umbilical vein endothelial cells and to evaluate the NF-κB in DVT inflammation-blood clots in the link of reaction function and Xiao Shuan Tong Mai(消栓通脉)intervention role. Methods With TNF-α induction human umbilical vein endothelial cells as experimental models, thirty rats were divided into the model group, Xiao Shuan Tong Mai(消栓通脉)high dose group, middle dose group and low dose group, The quantity changes of the NF-κB expression. RT-PCR and Western-Blot methods were used to detect. Results In human umbilical vein endothelial cells after TNF-αstimulation, the NF-κB expression level increased signifi⁃cantly higher than the cells unactivated NF-κB level. Under the role of serum containing Xiao Shuan Tong Mai (消栓通脉),NF-κB expression level dropped, and this inhibition was dose dependent effect. Under the role of high serum dose medication, the NF-κB expression level was minimal. Conclusion Xiao Shuan Tong Mai(消栓通脉)can inhibit the expression of NF-κB in activated human umbilical vein endothelial cells.关键词
人脐静脉血管内皮细胞/核转录因子-κB/消栓通脉颗粒Key words
Human umbilical vein endothelial cells/nuclear transcription factors-κB/Xiao Shuan Tong Mai granules分类
生物科学引用本文复制引用
阎伟,白增亮,侯玉芬..消栓通脉颗粒药物血清对TNF-α诱导人脐静脉内皮细胞NF-κB表达的影响[J].中国中西医结合外科杂志,2013,(1):40-42,3.基金项目
山东省自然基金课题(Y2008C148) （Y2008C148）
