中国中西医结合外科杂志Issue(4)：397-400,4.DOI:10.3969/j.issn.1007-6948.2013.04.015
香砂六君子汤对脾气虚证大鼠肠神经-ICC间信号转导通路损伤的作用
Expression Changes of Signal Transduction Pathway of Enteric Nerves-Interstitial Cells of Cajal in Rat with Deficiency of Spleen-Qi Syndrome and Therapeutic Effects of Xiang Sha Liu Jun Zi (香砂六君子) Decoction
摘要
Abstract
Objective To observe the expression changes of signal transduction pathway of enteric nerne system-interstitial cell (ENS-ICC) and the therapeutic effects of Xiang Sha Liu Jun Zi decoction(XSLJZD) in rats with deficiency of Spleen-Qi syndreme (DSQS). Methods One hundred Wistar rats were randomly divid-ed into control group, DSQS group, recovery group and XSLJZD group. The signal transduction pathway of ENS-ICC of the gastrointestinal tract was observed by using immunohistochemical double-staining with whole-amount preparation technique and confocal laser scanning microcopy. Results The IOD of ICC of intes-tine in DSQS group was significantly lower than that in control group(P<0.01), the ICC network was significant-ly disrupted. The IOD of ICC of intestine in the recovery group was slightly better than that in DSQS group(P<0.01), the ICC network was slightly recovered. The IOD of ICC of intestine in XSLJZD treatment group was sig-nificantly increased(P<0.01). After treatment with XSLJZD, the ICC network was significantly recovered. The IOD of protein kinase A(PKA)/protein kinase C(PKC) in DSQS group was significantly decreased(P<0.01). The signal transduction pathway was destroyed. The IOD of protein kinase A(PKA)/protein kinase C(PKC) in the re-covery group was slightly recovered(P<0.01). While the IOD of protein kinase A(PKA)/protein kinase C(PKC) in XSLJZD treatment group was significantly increased. The signal transduction pathway was significantly recov-ered. Conclusion XSLJZD can improve the gastrointestinal motility in DSQS and the mechanism may be re-lated to the repairing effect on the distribution and the connection of Signal Transduction Pathway of ENS-ICC in rat with DSQS.关键词
脾气虚证/香砂六君子汤/Cajal间质细胞/肠神经系统Key words
Syndrome of deficiency of spleen-qi/Xiang Sha Liu Jun Zi (香砂六君子) decoction/intersti-tial cells of Cajal/enteric nerve system分类
生物科学引用本文复制引用
丁伯龙,齐清会..香砂六君子汤对脾气虚证大鼠肠神经-ICC间信号转导通路损伤的作用[J].中国中西医结合外科杂志,2013,(4):397-400,4.基金项目
国家自然科学基金资助项目 （）
