中国中医药信息杂志Issue(8)：54-55,2.DOI:10.3969/j.issn.1005-5304.2013.08.019
RP-HPLC测定制萎扶胃丸中隐丹参酮和丹参酮ⅡA含量
Content Determination of TancantongⅡA and Cryptotanshinone in Zhiwei Fuwei Wan by RP-HPLC
刘效栓 1焦正花 1李喜香 1徐思羽1
作者信息
- 1. 甘肃省中医院药学部，甘肃 兰州 730050
- 折叠
摘要
Abstract
Objective To establish the method for determining the content of cryptotanshinone and tanshinone ⅡA in Zhiwei Fuwei Wan. Methods The determination of cryptotanshinone and tanshinone ⅡA was performed on Symmetry C18 column with mobile phase consisted of acetonitrile-0.2%phosphate (60∶40) at flow rate of 1.0 mL/min. The detection wavelength was set at 262 nm. Results The linear ranges of cryptotanshinone and tanshinone ⅡA were 0.081 4-0.407 2 µg (r=0.999 7) and 0.163 6-0.81 8 µg (r=0.999 5) respectively, the average recoveries were 99.70% (RSD=1.37%) and 99.22% (RSD=0.94%) respectively. Conclusion The method is simple and rapid for the quality control of the product.关键词
制萎扶胃丸/隐丹参酮/丹参酮ⅡA/反相高效液相色谱法Key words
Zhiwei Fuwei Wan/cryptotanshinone/Tanshinone ⅡA/RP-HPLC分类
医药卫生引用本文复制引用
刘效栓,焦正花,李喜香,徐思羽..RP-HPLC测定制萎扶胃丸中隐丹参酮和丹参酮ⅡA含量[J].中国中医药信息杂志,2013,(8):54-55,2.
