中国中医药信息杂志Issue(8)：66-68,3.DOI:10.3969/j.issn.1005-5304.2013.08.024
细胞膜色谱法与液相色谱-质谱联用筛选佛手的活性成分
Screening Effective Components from Fructus Citri Sarcodactylis by Cell Membrane Chromatography and HPLC/MS
摘要
Abstract
Objective To screen the active component from Fructus Citri Sarcodactylis which have aortic vascular relaxant effect. Methods The active components from Fructus Citri Sarcodactylis were screened by combining vascular smooth muscle/cell membrane chromatography (VSM/CMC) and liquid chromatography/mass spectrometry (LC/MS) with pharmacological assay in vitro. Results Bergapten was the active component of Fructus Citri Sarcodactylis that functions in vascular. The pharmacological experiment showed that bergapten had vasodilating effect. The capacity factor of this compound on the VSM/CMC model was found to be significantly correlated with its pharmacological effect. Conclusion Association between compound and membrane protein (receptor) can be reflected by using the VSM/CMC model. VSM/CMC-offline-LC/MS technology can be used to quickly screening new active components from natural medicine.关键词
细胞膜色谱法/液相色谱-质谱法/活性成分/佛手柑内酯Key words
cell membrane chromatography/LC/MS/active component/bergapten分类
医药卫生引用本文复制引用
王艳微,畅瑞苗,王新,岳媛,薛晖,李晓妮..细胞膜色谱法与液相色谱-质谱联用筛选佛手的活性成分[J].中国中医药信息杂志,2013,(8):66-68,3.基金项目
山西省科技厅社会发展项目(20120313016-4) （20120313016-4）
