中国中医药信息杂志Issue(9)：40-43,4.DOI:10.3969/j.issn.1005-5304.2013.09.014
小檗碱抗流感病毒性肺炎小鼠肺组织炎性损伤的实验研究
Resistance of Berberine on Lung Inflammatory Injury in Mice with Viral Pneumonia Caused by Influenza Virus
摘要
Abstract
Objective To observe the effects of berberine on ICAM-1, VCAM-1 expression and inflammatory cells exudation in mice with viral pneumonia caused by influenza virus, and explore its anti-injury effect. Methods Totally 108 BALB/c mice were randomly divided into control group, model group, and berberine group. 25 µL 50 LD50 influenza virus, mouse lung-adapted strain, was intranasally inoculated to model group and berberine group. 1 h after infection, control and model group were intragastrically given 25 µL distilled water, berberine group was treated by intraperitoneal injection with berberine at a dose of 0.005 g/(kg·d) for 5 days, twice per day. On day 2, 4 and 6 after infection, immunocytochemical method was used to detect ICAM-1 and VCAM-1 expression, and sorting cell count of leukocytes in bronchoalveolar lavage fluid (BALF) were counted. Results The expression of ICAM-1 and VCAM-1 in model group increased obviously on day 2, 4, 6, and which in berberine group decreased compared with model group (P<0.01). WBC, mononuclear cell, eosinophile cell and neutrophil cell number in model group increased significantly. WBC and neutrophil cell number decreased in berberine group on day 6 (P<0.01), and the mononuclear cell number decreased on day 4 (P<0.01). Conclusion Berberine inhibited the expression of ICAM-1 and VCAM-1, and decreased the inflammatory cells exudation in lung of mice with viral pneumonia caused by influenza virus. Berberine has protective effect on inflammatory injury of lung tissue in mice with viral pneumonia caused by influenza virus.关键词
小檗碱/病毒性肺炎/流感病毒/细胞间黏附分子-1/血管细胞黏附分子-1/炎性细胞/小鼠Key words
Berberine/viral pneumonia/influenza virus/ICAM-1/VCAM-1/inflammatory cell/mice分类
医药卫生引用本文复制引用
吴莹,李季倩,孟建,路广林,郝钰..小檗碱抗流感病毒性肺炎小鼠肺组织炎性损伤的实验研究[J].中国中医药信息杂志,2013,(9):40-43,4.基金项目
国家自然科学基金(30772871、81102697)；北京中医药大学创新团队项目(2011-CXTD-04) （30772871、81102697）
