中国中医药信息杂志2015，Vol.22Issue(11)：46-49,4.DOI:10.3969/j.issn.1005-5304.2015.11.013
何首乌及其成分对人正常肝细胞凋亡的影响
Effects of Polygoni Multiflori Radix and Its Three Ingredients on Human Normal Hepatocyte Apoptosis
摘要
Abstract
Objective To observe the effects of Polygoni Multiflori Radix on toxicity and apoptosis of human normal hepatocyte;To discuss the possible substances and channels of Polygoni Multiflori Radix to hepatic injury.Methods L02 cells were set as evaluation model. Cellular morphology in each group after medication was observed through Giemsa dyeing. Apoptosis rate and cell cycle of each group after medication were detected by FCM.Results Compared with blank control group, the 70% alcohol extracts of Polygoni Multiflori Radix (1, 2 and 4 mg raw materials per mL) had almost no effects on cellular morphology or cell cycle of L02 cells. Apoptosis rates after treatment with extracts of Polygoni Multiflori Radix were 9.51%, 10.3%, and 13.2% respectively. When L02 cells were treated with different dosages of emodin, rhein and gallic acid, three ingredients of Polygoni Multiflori Radix, apoptosis was obvious. The proportion of G1 phase cells significantly decreased, the S phase and G2 phase increased, which presented the concentration dependent effect. Apoptosis rates of three high dosages (100μmol/L) treated groups after treatment with emodin, rhein and gallic acid were 19.8%, 12.0%, and 13.7% respectively. Conclusion Polygoni Multiflori Radix has apoptosis-inducing effects on L02 cells. Apoptosis-inducing effects of its ingredient of emodin is stronger than rhein and gallic acid.关键词
何首乌/大黄素/大黄酸/没食子酸/肝细胞/凋亡/细胞周期Key words
Polygoni Multiflori Radix/emodin/rhein/gallic acid/hepatocyte/apoptosis/cell cycle分类
医药卫生引用本文复制引用
贾歌刘畅,庞晶瑶,柏兆方,牛明,赵艳玲,朱云,张雅铭,马致洁,赵奎君..何首乌及其成分对人正常肝细胞凋亡的影响[J].中国中医药信息杂志,2015,22(11):46-49,4.基金项目
国家自然科学基金(81373984) （81373984）
国家科技重大专项-重大新药创制(2015ZX09501-004-001-008) （2015ZX09501-004-001-008）
中医药行业科研专项(201507004-04) （201507004-04）
