中国中医药信息杂志2015，Vol.22Issue(11)：50-53,4.DOI:10.3969/j.issn.1005-5304.2015.11.014
归芪多糖对早衰WI-38细胞端粒酶活性的影响
Effects of Guiqi Polysaccharide on Telomerase Activity in Premature Senescence WI-38 Cells
摘要
Abstract
Objective To investigate the effects of Guiqi polysaccharide (GQP) on the telomerase activity in premature senescence of WI-38 cells;To discuss its mechanism of action.Methods MTT assay was used to observe the effects of different concentrations of H2O2 on the proliferation of WI-38 cells and screened the best concentration of H2O2 to induce premature senescence cellular model in WI-38 cells. ELISA and chemical staining method were used to evaluate the protection of GQP in telomerase activity and senescence-associated β-galactosidase activity in premature cellular senescence.Results 200μmol/L H2O2 treatment could induce premature senescence in WI-38 cells. There were no significant differences in senescence-associated β-galactosidase and telomerase activity between GQP group and normal control group (P>0.05), but had significant difference with model group (P<0.01).Conclusion GQP can increase the telomerase activity and inhibit the senescence-associated β-galactosidase activity in premature senescence of WI-38 cells.关键词
归芪多糖/过氧化氢/WI-38细胞/β-半乳糖苷酶/端粒酶Key words
Guiqi polysaccharide/H2O2/WI-38 cell/senescence-associatedβ-galactosidase activity/telomerase分类
医药卫生引用本文复制引用
蒲秀瑛,于双,樊文博,任菁,刘璐,马小龙,张伟杰..归芪多糖对早衰WI-38细胞端粒酶活性的影响[J].中国中医药信息杂志,2015,22(11):50-53,4.基金项目
国家自然科学基金(81260070) （81260070）
兰州市科技局项目(2011-1-71) （2011-1-71）
