HPLC同时测定紫朱软膏中左旋紫草素和β,β'-二甲基丙烯酰阿卡宁含量OACSCDCSTPCD
Simultaneous Determination of Contents of Shikonin and β,β'-dimethylacrylshikonin from Zizhu Ointment by HPLC
目的 建立同时测定紫朱软膏中紫草的左旋紫草素和β,β'-二甲基丙烯酰阿卡宁含量的高效液相色谱方法.方法 采用Welch Materials XB-C18色谱柱(4.6 mm×250 mm,18.5μm),流动相乙腈-水-甲酸(75:25:0.05),检测波长516 nm,柱温30 ℃,流速1.0 mL/min,进样量20μL,对紫朱软膏中紫草的有效成分左旋紫草素和β,β'-二甲基丙烯酰阿卡宁进行定量测定.结果 左旋紫草素在0.025 6~0.25…查看全部>>
Objective To establish an HPLC method to simultaneously determine the contents of shikonin andβ,β'-dimethylacrylshikonin inZizhu Ointment.Methods The contents of shikonin andβ,β'-dimethylacrylshikonin inZizhu Ointment were determined by HPLC. The chromatographic conditions were Welch Materials XB-C18 Column (4.6 mm × 250 mm, 18.5μm), with mobile phase of acetonitrile-water-formic acid (75:25:0.05), detection wavelength of 516 nm, temperature of 30℃, flow rat…查看全部>>
丁叶芳;李西林;柳国斌;张钰泉
上海中医药大学,上海 201203上海中医药大学,上海 201203上海中医药大学附属曙光医院,上海 201203上海中医药大学,上海 201203
医药卫生
紫朱软膏左旋紫草素β,β'-二甲基丙烯酰阿卡宁含量测定
Zizhu Ointmentshikoninββ'-dimethylacrylshikonincontent determination
《中国中医药信息杂志》 2015 (11)
76-78,3
上海市科委中药现代化专项(10DZ1972800)
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