中华耳科学杂志Issue(1)：107-111,5.DOI:10.3969/j.issn.1672-2922.2013.01.025
Mn-SOD在螺旋神经节区域分布差异与噪声性听力损伤的关系
Distribution of Mn-SOD in Spiral Ganglion Area and Noise-Induced Hearing Loss
摘要
Abstract
Objective To study distribution of Mn-SOD throughout the spiral ganglion and its correlation with the vulnerability of high-frequency hearing to noise induced damage. Methods Rats were divided into an experimental and a control group. The experimental animals were exposed to white noise at 115 dB SPL 2h/day for 3 days. Auditory brainstem responses (ABRs) were used to determine acoustic trauma in experimental animals. Immunohistochemical methods were used to identify the distribution of Mn-SOD in the spiral ganglion, before and after noise exposure. Activity levels of Mn-SOD in different regions of the spiral ganglion were assessd by xanthine oxidase method. Results (1) ABRs:, ABR thresholds at 4, 8, 16,20 and 32 kHz were significantly elevated 3 days after noise exposure, especially in the high-frequen⁃cy range. (2) Immunohistochemical staining: in normal spiral ganglions, the intensity of Mn-SOD immunoreactivity and numbers of immunopositive neurons appeared to be greater at the cochlear apex than at the base, with roughly intermediate findings in the middle turn. After noise exposure, the expression of Mn-SOD in the spiral ganglion was significantly higher than normal control group, especially at the apex. (3) Mn-SOD activity:After noise exposure, Mn-SOD activity levels de⁃clined compared with the control group, especially in the basal region. Conclusion These findings suggest that spiral gangli⁃on cells response to ROS exposure may vary along the cochlear spiral, and the difference in response capacity between the cochlear apex and base may explain the susceptibility to noise induced high-frequency hearing loss.关键词
噪声性聋/螺旋神经节/锰超氧化物歧化酶Key words
Noise-Induced Hearing Loss/Spiral Ganglion/Manganese Superoxide Dismutase分类
医药卫生引用本文复制引用
崔勇,何亚,武瑾,米文娟,何端军,张力鸥,邱建华..Mn-SOD在螺旋神经节区域分布差异与噪声性听力损伤的关系[J].中华耳科学杂志,2013,(1):107-111,5.基金项目
国际(地区)合作与交流项目(81120108008) （地区）
青年科学基金项目(81100713)资助 （81100713）
